GSE85589 ( circRNA )

cholangiocarcinoma ( cholangiocarcinoma )

Expression data from pancreatic cancers patients and healthy controls

Cancer : 101 / Control : 19

cel files were analysed and rma-normalized by using the Expression Console software

For the identification of miRNA biomarker of pancreatic cancer, total RNA containing miRNA was extracted from the serum samples using Affymetrix miRNA 4.0 and the serum miRNA purification kit (Genolution, Seoul, Korea)

Blood serum samples were extracted from 88 PC patients, 101 ICC patients, 7 stomach cancer (SC) patients, 5 colorectal cancer (CC) patients, 2 gastrointestinal stromal tumours (GIST) patients, 10 cholelithiasis patients, and 19 healthy subjects who had been clinically classified as so at the time of participation

Lee J, Lee HS, Park SB, Kim C et al. Identification of Circulating Serum miRNAs as Novel Biomarkers in Pancreatic Cancer Using a Penalized Algorithm. Int J Mol Sci 2021 Jan 20;22(3). PMID: 33498271

Total RNA containing miRNA was extracted from the serum samples using the Serum miRNA purification kit (Genolution, Seoul, Korea) according to the manufacturer’s instructions, and the RNA was resuspended with 12 μL of RNase-free water and stored at -80°C until microarray or qRT-PCR

total RNA

biotin

RNA samples (130 ng) were labelled using the FlashTag™ Biotin RNA Labeling Kit (Genisphere, Hatfield, PA)

labelled RNA was heated to 99°C for 5 minutes and then to 45°C for 5 minutes. RNA-array hybridization was performed with agitation at 60 rotations per minute for 16 hours at 48°C on an Affymetrix® 450 Fluidics Station

The chips were washed and stained using a Genechip Fluidics Station 450 (Affymetrix). The chips were then scanned using an Affymetrix GCS 3000 scanner