GSE41922 ( miRNA )

breast cancer ( breast )

Clinical Utility of Circulating MicroRNA Signatures for Breast Cancer Diagnosis (RT-PCR)

Cancer : 32 / Control : 22

Data was processed using GenEx software (Exiqon)

MicroRNAs from paired breast cancer tumours, normal tissue and serum samples derived from 32 patients were comprehensively profiled using microarrays (1300 microRNAs against tumour and normal tissues) or LNA RT-PCR panels (742 microRNAs against serum samples). Serum samples from healthy individuals (n=22) were also employed as normal controls. Significant serum microRNAs, identified by logistic regression, were validated in an independent set of serum samples from patients (n=82) and healthy controls (n=53).

Blood samples were collected in Becton Dickinson (Franklin Lakes, NJ) Vacutainer SST tubes. Serum was harvested by centrifugation at 2200g after allowing blood to clot for 30mins. 32 patient samples and 22 samples from healthy controls were obtained for profiling. Sera samples were stored at -80oC.

Chan M, Liaw CS, Ji SM, Tan HH et al. Identification of circulating microRNA signatures for breast cancer detection. Clin Cancer Res 2013 Aug 15;19(16):4477-87. PMID: 23797906

RNA was extracted using miRNeasy (Qiagen) following the manufacturer's recommendations.

total RNA

SYBR Green

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