GSE164174 ( miRNA )

gastric cancer ( gastric )

A novel combination of serum microRNAs for the detection of early gastric cancer

Cancer : 1423 / Control : 1417

The presence of miRNA was determined based on a corresponding microarray signal of greater than [the mean + 2 × standard deviation] of the negative controls signal, of which the top and bottom ranked ones by signal intensity were removed. Once a miRNA was considered present, the mean signal of the negative controls of which the top and bottom 5% ranked by signal intensity were removed was subtracted from the miRNA signal. When the signal value was negative (or undetected) after background subtraction, the value was replaced by 0.1 on a base 2 logarithm scale.

A serum miRNA combination could be a powerful classifier for the detection of patients with early stage gastric cancer.

Serum microRNA profiles of 2940 samples, which consist of 1423 gastric cancers, 1417 non-cancer controls, 50 esophageal cancers, and 50 colorectal cancers.

Abe S, Matsuzaki J, Sudo K, Oda I et al. A novel combination of serum microRNAs for the detection of early gastric cancer. Gastric Cancer 2021 Jul;24(4):835-843. PMID: 33743111

Total RNA was extracted each from 300uL serum samples using 3D-Gene® RNA extraction reagent from liquid sample kit (Toray Industries, Inc.).

total RNA

Cy5

miRNA was labeled using 3D-Gene® miRNA Labeling kit in accordance with the manufacturer's instructions.

Hybridized for 16 h at 32 ºC with rotary shaker (250 rpm). Hybridization buffer and washing protocol was attached in the 3D-Gene® miRNA oligo chip kit.

3D-Gene Scanner (Toray Industries, Inc.) was used for scanning. Images were quantified using Extraction version 2.0.0.6 (Toray Industries, Inc.).