GSE144521 ( lncRNA+mRNA )

cholangiocarcinoma ( cholangiocarcinoma )

RNA profiles in serum and urine extracellular vesicles of patients with cholangiocarcinoma mimicking tumor expression: novel liquid biopsy approach

Cancer : 35 / Control : 61

The data was preprocessed by Bioconductor lumi package (version 2.22.1). It was log2 transformed and quantile normalized. The normalized data table does not contain all the probes since the ones that do not follow the established criteria are filtered out. Only probes with a detection p-value lower than 0.01 in at least one sample are analzyed.

Cholangiocarcinoma (CCA) represents a heterogeneous group of biliary cancers with poor prognosis. Although the aetiology is generally unknown, factors like Primary Sclerosing Cholangitis (PSC) predispose to its development. Simultaneously, around 80% of patients with PSC have concomitant Ulcerative Colitis (UC).

Total RNA obtained from normal (NHC) and tumoral (EGI1 and TFK1) cholangiocyte cell lines and from extracellular vesicles (EVs) isolated from serum, urine and cholangiocyte cell line supernatant

Lapitz A, Arbelaiz A, O'Rourke CJ, Lavin JL et al. Patients with Cholangiocarcinoma Present Specific RNA Profiles in Serum and Urine Extracellular Vesicles Mirroring the Tumor Expression: Novel Liquid Biopsy Biomarkers for Disease Diagnosis. Cells 2020 Mar 14;9(3). PMID: 32183400

RNA was extracted from the described samples by using miRCURY RNA Isolation kit - Biofluids (EXIQON; ref #300112). Quality control was performed with 2100 Bioanalyzer.

total RNA

Biotin

Whole-Genome Gene Expression DASL®HT Assay Guide (Part#15018210 Rev. D)

Standard, as recommended by illumina

Standard, as recommended by illumina