GSE136321 ( miRNA )

prostate cancer ( prostate )

The identification of plasma exosomal miR-423-3p as a potential predictive biomarker for prostate cancer castration-resistance development by plasma exosomal miRNA sequencing

Cancer : 24 / Control : 24

Unaligned reads were trimmed and aligned against mirBase mature miRNA (Release 20) within Partek® Genomics Suite®. Samples were sequenced twice and read counts from two runs were combined. Genome_build: aligned against mirBase mature miRNA (Release 20)

Exosomal miRNAs play important roles in cancer progression and therapeutic resistance and have shown great potential as liquid biopsy biomarkers for cancer diagnosis/prognosis. We investigated for the first time plasma exosomal miRNAs for their association with and early detection potential of castration-resistant prostate cancer (CRPC). RNA-sequencing was performed to identify candidate exosomal miRNAs associated with development of CRPC in 24 treatment-naive prostate cancer (PCa) and 24 CRPC patients.

Examine differentially expressed plasma exosomal miRNAs between 24 treatment-naïve prostate cancer patients and 24 castration-resistant prostate cancer patients.

Guo T, Wang Y, Jia J, Mao X et al. The Identification of Plasma Exosomal miR-423-3p as a Potential Predictive Biomarker for Prostate Cancer Castration-Resistance Development by Plasma Exosomal miRNA Sequencing. Front Cell Dev Biol 2020;8:602493. PMID: 33490068

Plasma was isolated within 2 h of blood draw by centrifugation of the whole blood at 1200 g for 10 min, followed by another centrifugation of the supernatant at 2000 g for 10 min. The supernatant was taken as plasma and stored at -80°C for future use. 200 μl of plasma from each case was used. Prior to exosome precipitation, plasma samples were treated with RNase A to remove free circulating RNAs and then RNase A was inactivated with RNase inhibitor. Exosomes were isolated using the Total Exosome Isolation Kit (from plasma) (Invitrogen™) following the manufacturer’s instructions. Exosome pellet was re-suspended in Buffer RLT (Qiagen) with 0.25 μg/μl Proteinase K and incubated at 50°C for 30 min. Exosomal RNA was then extracted using miRNeasy Micro Kit (Qiagen) and QIAzol Lysis Reagent (Qiagen) according to the manufacturer’s protocols.

total RNA

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