GSE133684 ( lncRNA+mRNA )

pancreatic ductal adenocarcinoma ( pancreatic )

Blood extracellular vesicle long RNA sequencing of human blood from pancreatic ductal adenocarcinoma (PDAC), chronic pancreatitis (CP) and healthy individuals

Cancer : 284 / Control : 117

Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg38 whole genome using Hisat2. mRNA and lncRNA annotated in GencodeV25 were quantified using featureCounts

Blood samples were collected from all participants in 10-mL EDTA-coated Vacutainer tubes. Plasma was separated by centrifugation at 3,000 rpm for 10 min at room temperature (25°C) within 2 h after blood collection and then centrifuged at 13,000 rpm for 10 min at 4°C to remove debris. Isolated plasma samples were stored at -80°C until use. EV RNA were isolated by affinity-based binding to spin columns using an exoRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany). RNA libraries were prepared for sequencing using SMARTer® Stranded Total RNA-Seq Kit - Pico Input Mammalian. RNA-seq was performed by the Illumina sequencing platform on a 150-bp paired-end run.

Blood extracellular vesicle long RNA profiles of human plasma by deep sequencing using Illumina Hiseq.

Yu S, Li Y, Liao Z, Wang Z et al. Plasma extracellular vesicle long RNA profiling identifies a diagnostic signature for the detection of pancreatic ductal adenocarcinoma. Gut 2020 Mar;69(3):540-550. PMID: 31562239

RNA libraries were prepared for sequencing using SMARTer® Stranded Total RNA-Seq Kit - Pico Input Mammalian. Plasma EV RNAs were extracted by exoRNeasy Serum/Plasma kit.

total RNA

n/a

n/a

n/a

n/a