GSE112840 ( miRNA )

Esophageal Squamous Cell Carcinoma ( esophageal )

Microarray expression profile of micro RNAs in serum for Esophageal Squamous Cell Carcinoma

Cancer : 52 / Control : 52

Raw data were normalized by Quantile algorithm, included in the R package AgiMicroRna(López-Romero, P. BMC Genomics. 2011.).

The purpose of this study is to explore the miRNAs expression profiles in the serum from esophageal squamous cell carcinoma (ESCC) patients.

We have completed the Arraystar Human miRNA Array analysis of the 104 serum samples from preoperative ESCC patients (n=52) and healthy controls (n=52).

Zheng D, Ding Y, Ma Q, Zhao L et al. Identification of Serum MicroRNAs as Novel Biomarkers in Esophageal Squamous Cell Carcinoma Using Feature Selection Algorithms. Front Oncol 2018;8:674. PMID: 30719423

Total RNA was extracted and purified using miRNeasy Serum/Plasma Kit (Cat # 217184, Qiagen, German), following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).

total RNA

Cy3

miRNA molecular in total RNA was labeled by miRNA Complete Labeling and Hyb Kit (Cat # 5190-0456, Agilent technologies, Santa Clara, CA, US) followed the manufacturer’s instructions, labeling section.

Each slide was hybridized with 100ng Cy3-labeled RNA using miRNA Complete Labeling and Hyb Kit (Cat # 5190-0456, Agilent technologies, Santa Clara, CA, US) in hybridization Oven (Cat # G2545A, Agilent technologies, Santa Clara, CA, US) at 55°C，20 rpm for 20 hours according to the manufacturer’s instructions, hybridization section. After hybridization, slides were washed in staining dishes (Cat # 121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit (Cat #5188-5327, Agilent technologies, Santa Clara, CA, US).buffer was added to dilute the labeled cRNA. 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the circRNA expression microarray slide. The slides were incubated for 17 hours at 65°C in an Agilent Hybridization Oven

Slides were scanned by Agilent Microarray Scanner (Cat # G2565CA, Agilent technologies, Santa Clara, CA, US) and Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US) with default settings.